Journal: Frontiers in Immunology
Article Title: Nrf2/HO-1–dependent inhibition of ferroptosis underlies the antioxidant effects of 5-O-methylvisammioside in colitis
doi: 10.3389/fimmu.2025.1738408
Figure Lengend Snippet: CCK-8 Assay, Establishment of a Caco-2 Cell Monolayer Model;the Effects of MeV on Barrier Function and Inflammatory Response. (A) CCK8 analysis. (B, C) TEER value and ALP activity of monolayer cell model. (D) level of inflammatory factors. (E, F) Western blotting and RT-qPCR analyses of occuldin and ZO-1. Data are presented as mean ± SD. #P<0.05, ## p < 0.01, ###p < 0.001 versus CON group; *p < 0.05, and ***p < 0.001 versus Erastin group. ▴P<0.05, ▴▴p < 0.01, ▴▴▴p < 0.001 versus Fer-1 group.
Article Snippet: The Erastin and Fer-1 were acquired from MedChemExpress (Shanghai, China).
Techniques: CCK-8 Assay, Activity Assay, Western Blot, Quantitative RT-PCR